Candida parapsilosis culture extracts: in vitro, antagonistic action against Candida albicans, Candida auris and Candida parapsilosis

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Published: Nov 29, 2022
DOI: https://doi.org/10.52600/2763-583X.bjcr.2023.3.1.24-29
Keywords:
Fungal infections, Candida spp, antifungal activity

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Thiago Henrique Lemes
São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences
Julyanna Andrade Silva Nascentes
São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences
Luis Octávio Regasini
São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto, Brazil
Taiza Maschio-Lima
São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto, Brazil
Bianca Gottardo de Almeida
São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences
Mariela Domiciano Ribeiro
São José do Rio Preto School of Medicine (FAMERP), Departament of Dermatological, Infectious, and Parasitic Diseases
Veridianna Camilo Pattini
São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences
Leticia de Queiroz Mozaner
São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences
João Paulo Zen Siqueira
São José do Rio Preto School of Medicine (FAMERP), Dept. of Dermatological, Infectious, and Parasitic Diseases
Margarete Teresa Gottardo de Almeida
São José do Rio Preto School of Medicine (FAMERP), Dept. of Dermatological, Infectious, and Parasitic Diseases

Abstract

Fungal infections caused by Candida species has increased significantly in recent years. Additionally, resistance to conventional therapy is an aggravating factor causing high morbidity and mortality, especially in immunocompromised patients or those undergoing treatment with another antimicrobials. During the infection process, Candida species produces metabolites which can self-regulate population density, in addition to controlling several virulence factors and exerting action on other microorganisms. In view of this fact, the present study evaluated the in vitro antifungal activity of the pure culture extract of Candida parapsilosis against Candida albicans, Candida auris and Candida parapsilosis. Fungal culture extracts of C. parapsilosis were prepared in Sabouraud Dextrose Broth, extracted with ethyl acetate and dried in a rotary evaporator. Subsequently, tests were performed to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). The antifungal activity of the extracts against Candida species showed values of MIC ranging between 500 - 2000 µg/mL and a MFC range of 1414 µg/mL - 2000 µg/mL. Future investigations for the identification and composition of this fungal culture extract will provide insights about metabolites are present and involved in the antifungal activity shown here, contributing for the future to new therapeutic approaches in the control of infections caused by Candida.

Article Details

How to Cite
Lemes, T. H., Nascentes, J. A. S. ., Regasini, L. O., Maschio-Lima, T., de Almeida, B. G. ., Domiciano Ribeiro, M., Pattini, V. C., Mozaner, L. de Q. ., Siqueira, J. P. Z., & de Almeida, M. T. G. (2022). Candida parapsilosis culture extracts: in vitro, antagonistic action against Candida albicans, Candida auris and Candida parapsilosis. Brazilian Journal of Case Reports, 3(1), 24–29. https://doi.org/10.52600/2763-583X.bjcr.2023.3.1.24-29
Issue
Vol. 3 No. 1 (2023): January/March - 2023
Section
Experimental Reports
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Author Biographies

Thiago Henrique Lemes, São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences

São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto, Brazil.

Julyanna Andrade Silva Nascentes, São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences

São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto, Brazil.

Luis Octávio Regasini, São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto, Brazil

São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto, Brazil.

Taiza Maschio-Lima, São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto, Brazil

São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto, Brazil.

Bianca Gottardo de Almeida, São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences

São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto, Brazil.

Mariela Domiciano Ribeiro, São José do Rio Preto School of Medicine (FAMERP), Departament of Dermatological, Infectious, and Parasitic Diseases

São José do Rio Preto School of Medicine (FAMERP), Department of Dermatological, Infectious, and Parasitic Diseases São José do Rio Preto, Brazil.

Veridianna Camilo Pattini, São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences

São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto, Brazil.

Leticia de Queiroz Mozaner, São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences

São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto, Brazil.

João Paulo Zen Siqueira, São José do Rio Preto School of Medicine (FAMERP), Dept. of Dermatological, Infectious, and Parasitic Diseases

São José do Rio Preto School of Medicine (FAMERP), Department of Dermatological, Infectious, and Parasitic Diseases São José do Rio Preto, Brazil.

Margarete Teresa Gottardo de Almeida, São José do Rio Preto School of Medicine (FAMERP), Dept. of Dermatological, Infectious, and Parasitic Diseases

São José do Rio Preto School of Medicine (FAMERP), Department of Dermatological, Infectious, and Parasitic Diseases São José do Rio Preto, Brazil.

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